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. 2017 Mar 3;16:8. doi: 10.1186/s12941-017-0182-4

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — SDS-PAGE analysis of recombinant VirB12 protein expression and its purification. Lane 1 elution of recombinant VirB12 protein through Ni-NTA column. Lane 2 protein molecular mass marker (KDa). Lane 3 protein protein expression in transformed Bl21 (DE3) with pET28a-VirB12 recombinant vector 3 h after adding IPTG