Table 1.
Proteins identified in methanol extract by LC-MS/MS. Proteins with a single peptide are considered “tentatively identified”
| Accession | Protein | MW [kDa] | Scores | # Peptides | |
|---|---|---|---|---|---|
| 1 | ENSGALP00000036403 | Ovalbumin | 42.9 | 629.9 | 11 |
| 2 | ENSGALP00000005544 | Ovomucoid | 22.6 | 338.1 | 7 |
| 3 | ENSGALP00000017755 | Ovocleidin-116 | 76.8 | 225 | 2 |
| 4 | ENSGALP00000008163 | Orosomucoid 1 (ovoglycoprotein) precursor | 22.3 | 167 | 3 |
| 5 | ENSGALP00000042635 | peptidyl-prolyl cis-trans isomerase FKBP1A | 8.9 | 163.5 | 3 |
| 6 | ENSGALP00000016177 | Lysozyme C | 16.2 | 129.2 | 2 |
| 7 | ENSGALP00000016632 | keratin 8, type II | 42.1 | 119.1 | 2 |
| 8 | ENSGALP00000031725 | SH3 domain binding glutamic acid-rich protein like (SH3BGRL), mRNA | 12.9 | 109.6 | 2 |
| 9 | ENSGALP00000027483 | Ubiquitin-fold modifier 1 | 9 | 102.1 | 1 |
| 10 | ENSGALP00000035930 | Gallinacin-9 | 7.3 | 85.7 | 2 |
| 11 | ENSGALP00000010763 | Ovocalyxin-36 precursor | 58.3 | 83.7 | 1 |
| 12 | ENSGALP00000019758 | Diazepam binding inhibitor (GABA receptor modulator, acyl-CoA binding protein) (DBI), mRNA. | 9.6 | 69.8 | 2 |
| 13 | ENSGALP00000026846 | Gallinacin-10 | 7.1 | 62.6 | 2 |
| 14 | ENSGALP00000006093 | Keratin, type I cytoskeletal 19 | 46 | 62.3 | 3 |
| 15 | ENSGALP00000009976 | Usher syndrome 1C | 100.1 | 51.9 | 1 |
| 16 | ENSGALP00000012729 | Uncharacterized protein | 21 | 50.9 | 1 |
| 17 | ENSGALP00000040654 | Thymosin, beta 4 | 5 | 47.8 | 2 |
| 18 | ENSGALP00000043411 | Collagen, type XVI, alpha 1 | 156.6 | 42 | 1 |
| 19 | ENSGALP00000002523 | Signal peptidase complex subunit 1 homolog | 27.2 | 37.9 | 1 |
| 20 | ENSGALP00000013908 | Zinc finger BED domain-containing protein 4 | 132.4 | 37.8 | 2 |
| 21 | ENSGALP00000038912 | Alpha-D-globin (HBAD), mRNA. | 15.7 | 37.3 | 1 |
| 22 | ENSGALP00000019988 | Utrophin | 398.6 | 36.5 | 2 |
| 23 | ENSGALP00000000876 | Fatty acid-binding protein, heart | 14.8 | 34.6 | 1 |
| 24 | ENSGALP00000026777 | Nociceptin precursor | 21.4 | 34.4 | 1 |
| 25 | ENSGALP00000026863 | Polycystic kidney and hepatic disease 1 (autosomal recessive) | 440 | 32.7 | 1 |
| 26 | ENSGALP00000018601 | Serine peptidase inhibitor, Kazal type 2 (acrosin-trypsin inhibitor) | 6 | 26.7 | 1 |
| 27 | ENSGALP00000025439 | Probable arginyl-tRNA synthetase, mitochondrial | 65.3 | 25.2 | 1 |
| 28 | ENSGALP00000039913 | Transforming, acidic coiled-coil containing protein 1 | 86.4 | 24.5 | 2 |
| 29 | ENSMGAP00000001722 | elaC ribonuclease Z 2 | 94.2 | 24.2 | 1 |
| 30 | ENSGALP00000031519 | Fibroblast growth factor 2 | 16.2 | 22.8 | 1 |
| 31 | ENSGALP00000039675 | Nuclear receptor coactivator 2 | 44.7 | 21.5 | 1 |
| 32 | ENSGALP00000000325 | WD repeat-containing protein 36 | 98.2 | 21.2 | 1 |
| 33 | ENSGALP00000019412 | Septin 3 | 40 | 20.5 | 1 |
| 34 | ENSGALP00000027541 | High mobility group protein B1 | 24.9 | 20 | 1 |
| 35 | ENSGALP00000014919 | tRNA (adenine-N(1)-)-methyltransferase non-catalytic subunit TRM6 | 54.2 | 19.8 | 1 |
| 36 | ENSGALP00000043133 | Fibrinogen silencer binding protein | 36.4 | 19 | 1 |
| 37 | ENSGALP00000000726 | SH3 domain binding glutamate-rich protein like 3 | 10.5 | 18.8 | 1 |
| 38 | ENSGALP00000038283 | UPF3 regulator of nonsense transcripts homolog B (yeast) | 56.9 | 18.4 | 1 |
| 39 | ENSGALP00000000275 | Mutated in colorectal cancers | 112.6 | 18.3 | 1 |
| 40 | ENSGALP00000038735 | Polyubiquitin-B Ubiquitin | 109.6 | 15.5 | 1 |
| 41 | ENSGALP00000040476 | Neuregulin 2 | 65.5 | 15 | 1 |
The underlined proteins also occur in guanidine HCl extract (Additional file 1: Table S1)