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. 2016 Nov 29;129(8):950–958. doi: 10.1182/blood-2016-06-720649

Figure 4.

Figure 4.

CYTH1 knockdown affects CD34+CD38− cell mobility and localization within the marrow space. (A) Overview of intravital imaging to visualize lodgment of SCR and CYTH1-sh1 transduced CD34+CD38− cells. On the right, representative stills from intravital imaging of CD34+CD38− cells transduced with SCR (left) or CYTH1-sh1 (right). Green (carboxyfluorescein diacetate succinimidyl ester [CFSE]), cell; cyan (second harmonic signal), bone; magenta (Quantum Dots −655), endothelium. The scale bar represents 50 μm. (B) Displacement of the cells in calvaria bone (n = 3). (C) Migration speed of the cells in calvaria bone (n = 3). (D) Sphericity of the cells migrating within calvaria bone (n = 3). (E) Cell distance from the bone surface and endothelium (EC) (n = 3).