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. Author manuscript; available in PMC: 2017 Mar 3.
Published in final edited form as: Biochemistry. 2015 Aug 10;54(32):4987–4997. doi: 10.1021/acs.biochem.5b00502

Figure 7. Metal binding to histidine-lacking heterotrimers.

Figure 7

(A) CD temperature melting curves monitored at 223nm of 0.18mM A:B:C heterotrimer peptide in 10mm Tris buffer, pH=7.4, 150mM NaCl in (red) no metal (green) 0.36mM ZnCl2 , (blue) 0.36mM CuCl2 (B) their respective first derivative plots.