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. Author manuscript; available in PMC: 2018 Mar 2.
Published in final edited form as: Curr Protoc Chem Biol. 2017 Mar 2;9(1):11–37. doi: 10.1002/cpch.17

Figure 2.

Figure 2

Chemoenzymatic synthesis of Ub~MES. Ubiquitin is incubated with E1 enzyme in the presence of ATP and 100 mM 2-mercaptoethanesulfonic acid sodium salt (MESNa). The initially formed E1~Ub thioester reacts with MESNa, producing Ub~MES and regenerated E1 enzyme.