Table 2.
Homologous genes for sulfur oxidation enzymes found on the T. thermophila genome. Genbank accession number for sequence with highest similarity is shown with corresponding e-value/% similarity in brackets. Known function is included with references to protein families, if known.
| Protein | Node#/p osition in node |
Highest sequence similarity |
Nearest neighbor | comments |
|---|---|---|---|---|
| SoxA | 1 (241316–242146) |
YP_390871.1 (1e-180/85.51%) |
T. crunogena XCL-2 | Sulfur oxidation protein with signal peptide and conserved cytochrome c |
| SoxZ | 1 (242251–242589) |
YP_390872.1 (1e-62/89.81%) |
T. crunogena XCL-2 | Sulfur oxidation protein (pfam08770) |
| SoxY | 1 (242629–243096) |
YP_390873.1 (6e-86/91.61%) |
T. crunogena XCL-2 | Sulfur oxidation protein with signal peptide |
| SoxX | 1 (243134–243508) |
YP_390874.1 (2e-81/93.55%) |
T. crunogena XCL-2 | Cytochrome c with signal peptide |
| Cytochrome c | 1 (267914–268273) |
YP_390898.1 (3e-57/86.00%) |
T. crunogena XCL-2 | Cytochrome c553 with signal peptide |
| Hypothetical protein (SoxA) | 6 (119648–120526) |
YP_003644808.1 (2e-128/66.41%) |
Thiomonas intermedia | Sulfur oxidation with non-matching signal peptide (KEGG Adeh_2281; KEGG Anaek_1594) |
| Hypothetical protein (SoxX) | 6 (120852–121649) |
YP_003644809.1 (4e-38/61.50%) |
Thiomonas intermedia K12 | Sulfur oxidation protein with cytochrome c |
| rhodanese | 6 (165983–166432) |
WP_011371372.1 (3e-83/85.71%) |
T. crunogena | sulfurtransferase |
| SoxB | 5 (107062–108993) |
YP_391815.1 (0.0/79.06%) |
T. crunogena XCL-2 | Sulfate thiol esterase UshA with signal peptide, SoxB (cd07411) with metallophosphatase binding site, C-terminal domain (pfam02872) |
| Sulfide dehydro-genase | 5 (129755–131155) |
WP_019591503.1 (3e-139/52.18%) |
Thioalkalivibrio sp. ALE20 | NAD(FAD) dependent dehydrogenase with NAD(P) binding domain (cl17500) and flavocytochrome c sulfide dehydrogenase (pfam204176) |
| SoxC | 10 (68564–69925) |
YP_390426.1 (0.0/94.48%) |
T. crunogena XCL-2 | Sulfite oxidase with molydopterin-binding domain (cl00199) |
| SoxD | 10 (69906–70994) |
YP_390427.1 (0.0/88.69%) WP_020286444.1 (7e-34/38.89%) |
T. crunogena XCL-2 Osedax symbiont Rs2 |
Hypothetical protein containing cytochrome c (pfam00034) Diheme cytochrome c SoxD (pfam00034) |
| Sulfide dehydro-genase | 9 (44242–45666) |
WP_019896075.1 (0.0/79.64%) YP_001997639.1 (2e-128/47.10%) |
T. halophila Chlorobaculum parvum NCIB 8327 |
Hypothetical protein with pyridine nucleotide disulfide oxidoreductase (pfam07992) Flavocytochrome c sulfide dehydrogenase (pfam09242) |
| Sulfite reductase | 3 (111050–112147) |
YP_392157.1 (0.0/77.81%) |
T. crunogena XCL-2 | Oxidoreductase containing FAD binding domain (pfam00667), cytochrome p450 – sulfite reductase (NADPH dependent reduction of SO3 to H2S) |
| Sulfite reductase | 4 (51414–53828) |
WP_004280698.1 (0.0/39.79%) |
Acinetobacter lwoffii | Fe-regulated membrane protein PiuB containing CysJ sulfite reductase and flavoprotein with cytochrome p450 – sulfite reductase |
| Sulfide-quinone reductase | 2 (33149–34273) |
YP_391650.1 (0.0/85.29%) |
T. crunogena XCL-2 | Sulfide-quinone reductase containing pyridine nucleotide – disulfide oxidoreductase (pfam07992) |
| Thiosulfate dehydro-genase | 16 (13271–14293) |
WP_019556080.1 (9e-142/58.88%) WP_012969337.1 (8e-41/39.41%) |
T. arctica Allochromatium vinosum DSM180 |
Hypothetical protein – haem binding cytochrome c (pfam00034) Thiosulfate dehydrogenase containing different signal peptide |
| rhodanese | 13 (55570–55866) |
YP_391186 (2e-63/95.88%) |
T. crunogena | Rhodanese-like protein |
| rhodanese (SoxL) | 19 (25750–26430) |
YP_003262911 (6e-108/72.64%) YP_003444124 (7e-59/46.23%) |
Halothiobacillus neapolitanus c2 Allochromatium vinosum DSM 180 |
Rhodanese (pfam PF00581) Sulfurtransferase (SoxL) |