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. Author manuscript; available in PMC: 2018 Feb 15.
Published in final edited form as: J Am Chem Soc. 2017 Feb 2;139(6):2520–2528. doi: 10.1021/jacs.7b00154

Figure 4.

Figure 4

Relative QY changes on binding of SRA to HM and NM duplexes labeled by (a) 3HCnt or (b) thG. QYHM/QYNM corresponds to the ratio of the QY of 3HCnt/thG in the SRA/HM-duplex complex to that in the SRA/NM-duplex. Concentration of labeled duplexes was 1 μM, while the SRA concentration was chosen to complex at least 80% of the DNA molecules (Tables S5 and S6). Data are expressed as means ± standard deviation for n = 5 independent experiments. Experiments were done in phosphate buffer 20 mM, NaCl 50 mM, TCEP 2.5 mM, 1 mM EDTA, PEG 0.05%, pH 7.5, at T = 20 °C.