Figure 4.

Schematic representation of synthesis of protein imprinted polymers on CS microsphere using immobilized protein as a template. The synthesis involved three steps; Firstly, template BSA was covalently immobilized on the polysaccharide core by forming imine bonds. The second step involved siloxanes polymerization on the polysaccharide–protein surface. It resulted in a polymeric network molded around the template. The template protein was removed in the third step. Cavities complementary to the template protein in shape, size, and functional group orientation were created (reproduced from [56] with permission).