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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Calibration free concentration analysis using SPR. l-selectin LE-His in cell culture supernatant was analyzed in duplicate. The sensorgrams (colored lines) and the fits (black lines) at the different flowrates are shown.