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Affinity matrix performance. (a) amount of l-sel LE-His from cell culture supernatant bound to 1 eq. Ni-NTA or 1 eq. aptamer LD201mΔ1 (ELISA data) (b) yield calculated by the total amount of l-sel LE-His in 150 mL cell culture supernatant (988 ± 276 µg); ±SD from N = 2 in duplicates.
| Affinity Matrix | l-sel LE-His (µg) (a) | Yield (%) (b) |
|---|---|---|
| Ni-NTA | 167 ± 21 | 17 |
| LD201mΔ1 | 608 ± 23 | 62 |
