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. 2017 Jan 10;292(8):3089–3098. doi: 10.1074/jbc.M116.769703

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© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 8. — Bilin transfer assays employing ΦCpeT and PmCpeB in combination with PmCpeS. The solid spectrum shows fluorescence emission of the ΦCpeT·PEB complex incubated with purified recombinant PmCpeB. The obtained fluorescence emission is solely due the ΦCpeT·PEB complex and no transfer to PmCpeB is observed; addition of purified recombinant host PBP lyase CpeS after 15 min is shown as dotted spectrum (···). After 30 min incubation the spectrum shown in dashes is observed (- - -). The additional fluorescence emission at 568 nm is due to the assembled PmCpeB (15). See text for details.