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NMIIA is required for upstream signaling in focal adhesion maturation. A, COS-7 cells carrying indicated plasmid constructs were allowed to spread on fibronectin-coated cover glass for 60 min and then harvested for Western blotting analysis with indicated antibodies. B, MDA-MB-231 cells were subjected to lentivirus-based shRNA depletion of NMIIA. The shRNA cells were then transfected with indicated NMIIA constructs (right two lanes) and subjected to spreading analysis as in A.
