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Deletion of all three Raf isoforms impairs chondrocyte proliferation. A, BrdU immunostaining of growth plates of E18.5 control and triple KO embryos. Graph indicates the percentage of BrdU-positive chondrocytes in the proliferating layer of tibial growth plates (*, p = 0.04). Data are representative of four mice per genotype. B, evaluation of gene expression by quantitative RT-PCR. Data are representative of four mice per genotype. Error bars indicate ± S.E. *, p < 0.025.
