FIGURE 6.

TLP suppresses tumor growth. A, left, a representative photograph of xenograft tumor-bearing mice at 25 days after injection of shCtrl and shTLP-expressing HCT116 cells. Right, weight of each tumor was plotted. **, p < 0.01 (Welch's t test). B, point mutation map of human cancers obtained from The cBio Cancer Genomics Portal. TLP contains two TBP-homology domains (TBP). Mutations at the 99th Glu are scored as D99E and D99H, and the D99H mutant was used in this study. The binding region of p53 (p53 binding: amino acid residue between the 64th and 115th) is depicted as an orange solid line. C, glutathione-Sepharose-bound GST-p53 and FH-TLP (WT) or D99H were mixed, and p53-bound TLP was detected. D, WT and p53-null HCT116 cells were transfected with a mouse TLP expression vector. Forty-eight hours after transfection cell numbers were counted. Results are presented as cell numbers relative to control cells (mean ± S.D.). **, p < 0.01; NS, not significant (Welch's t test). E, HCT116 cells were transfected with wild-type TLP or D99H. Twenty-four hours after transfection the numbers of viable cells were counted at the indicated culture times (mean ± S.D.). Statistical analysis was performed between the values of wild-type TLP and that of D99H. *, p < 0.05; **, p < 0.01 (Welch's t test).