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. 2016 Dec 12;292(8):3224–3238. doi: 10.1074/jbc.M116.760132

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FIGURE 7. — Product distribution after in vivo conversion of methylglyoxal in GLO1^−/− Schwann cells is in the favor of lactaldehyde. A, representative chromatogram of intracellular d₄ products in wild-type Schwann cells 60 min after the exposure to d₄-MG. Chemical structure displays the complex of labeled d₄-d-lactate/d₄-hydroxyacetone and the derivatizing agent (PFBHA) B, representative chromatogram of intracellular d₄ products in GLO1^−/− Schwann cells 60 min after the exposure to d₄-MG. Chemical structure displays the complex of labeled d₄-lactaldehyde/d₄-hydroxyacetone and the derivatizing agent (PFBHA) C, product distribution of isotopically labeled intracellular metabolites in percent after the conversion of d₄-MG in vivo. Wild-type and GLO1^−/− Schwann cells were exposed to isotopically labeled d₄-MG (100 μm) for 60 min, and products were analyzed by LC-MS/MS.