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. 2017 Jan 5;292(8):3252–3261. doi: 10.1074/jbc.M116.765081

FIGURE 1.

FIGURE 1.

The porP, porK, porL, porM, and porN genes are co-transcribed. A, schematic representation of the P. gingivalis porP-K-L-M-N fragment (PGN_1677 to PGN_1673). The fragments corresponding to gene junctions and amplified in the RT-PCR experiments are indicated below (PK, 393 bp; KL, 377 bp; LM, 423 bp; MN, 432 bp). B, operon structure of the P. gingivalis porP-K-L-M-N fragment. Shown are agarose gel analyses of the indicated gene junctions amplified by PCR from cDNA (top panel), genomic DNA (center panel, positive control), and total RNA (bottom panel, negative control). The presence of a PCR fragment in the cDNA gel demonstrates co-transcription of the genes located 5′ and 3′ of the amplified region. Molecular weight markers (in kilobases) are indicated on the left.