FIGURE 4.

Oligomeric state of the PorL, PorM, and PorN soluble domains. A–C, the purified PorL cytoplasmic domain (PorL_C, A), PorM periplasmic domain (PorM_P, B), and the PorN protein (C) shown in the left panels (Coomassie Blue staining) were subjected to MALS/quasi-elastic light scattering/UV/refractive index analyses (right panels). The molecular weights of the proteins or complexes are indicated below their corresponding peaks. The absorbance at λ = 260 nm (in absorbance units, AU) and the molecular mass (in grams per milliliter) are plotted versus the volume of elution (in minutes).