FIGURE 5.

Carbonate extractions at high pH of membrane-associated POMP10-GFP fusion proteins. Immunoblots of equal cells equivalents of total (T), pellet (P), and supernatant (S) fractions of a carbonate-extracted crude mitochondrial pellet obtained by digitonin extraction of cells expressing the indicated POMP10-GFP variants (A–E). The extraction was performed at both pH 10.8 and pH 11.5, and immunoblots were analyzed by anti-GFP. ATOM40 and Cyt c served as markers representing an integral and peripheral membrane protein, respectively.