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Structural model of ExoU. Shown is a model based on PDB 4AKX (13) displaying the catalytic domain (cyan), bridging domain (brown), and C-terminal four-helix bundle (red/yellow). Native side chains at sites of spin label attachment are shown in yellow. The catalytic serine (Ser-142) is indicated by magenta spheres. Loops were modeled using Rosetta (see “Experimental Procedures”).
