FIGURE 9.

PP2A-FoxO1-Smad3 complex formation. a, Western blotting analysis of Smad3-AMPKα-FoxO1 proteins in cytoplasmic and nuclear fractions of HepG2 cells with and without TGF-β1 treatments. b, co-immunoprecipitation assay using anti-FoxO1 as the primary immunoprecipitation antibody (IP) and cytoplasmic and nuclear fractions of HepG2 cells treated with TGF-β1 ligand and SB431542 followed by Western blotting (WB) analyses of p-Smad3, total Smad3, and PP2A (a representative blot from two independent experiments). Anti-tubulin and anti-histone H3 antibodies were used as the internal control for the cytoplasmic and nuclear fractions, respectively.