TABLE 2.

Design of full sequence-wide gene deletions in plasmid pCT281 of B. thuringiensis CT-43

The full sequence of plasmid pCT281 (∼281 kb) was divided into 28 regions (every 10 kb from 0 to 281 kb). Primer locations in each region defined the deleted sequence range, within which certain genes that were functionally unidentified or predicted to be biosynthesis-related were targeted and described according to the National Center for Biotechnology Information (NCBI) database. The primer locations in blue and red represent the completed constructions of gene-knockout recombinant vectors and the further completed transformations of relevant vectors into strain CT-43, respectively. The targeted genes in green were successfully deleted genes.

^a No genes were targeted in this region.