TABLE 4.

Bacterial strains and plasmids used in this study

Strain and plasmid	Description	Source or reference
DH5α	E. coli cloning host	Ref. 63
Rosetta-TbrA	E. coli Rosetta expressing the TbrA protein	This work
Rosetta-pET28a	E. coli Rosetta with empty vector pET28a	This work
CT-43	B. thuringiensis, no flagellum; TAA and thuringiensin producer	Refs. 34 and 35
CT-43-55	Strain CT-43 derivative; TAA producer	Ref. 35
CT-43-62	Strain CT-43 derivative; TAA producer	Ref. 35
CT-43-7	Strain CT-43 derivative; thuringiensin producer	Ref. 35
CT-43-1c	Strain CT-43 derivative; thuringiensin producer	Ref. 35
F15	Strain CT-43 derivative; no TAA and thuringiensin production	This work
BMB171	Acrystalliferous mutant of B. thuringiensis	Ref. 48
BMB2451	BMB171 with plasmid pBMB2451	This work
BMB2451-ΔtbrA	BMB171 with plasmid pBMB2451-ΔtbrA	This work
BMB2451-ΔtbrB	BMB171 with plasmid pBMB2451-ΔtbrB	This work
BMB2451-ΔtbrA:tbrA	BMB171 with plasmids pBMB2451-ΔtbrA and pEMB0603-tbrA	This work
BMB2451-ΔtbrB:tbrB	BMB171 with plasmids pBMB2451-ΔtbrB and pEMB0603-tbrB	This work
BMB-TbrB-GFP	BMB171 with plasmid pBMB-tbrB-gfp	This work
BMB-GFP	BMB171 with plasmid pBMB-gfp	This work
BMB2451-tbrB	BMB2451 with plasmid pEMB0603-tbrB	This work
pHT304	E. coli–B. thuringiensis shuttle vector	Ref. 47
pHT304-TS	pHT304 derivative with a temperature-sensitive Bacillus replicon	Ref. 21
pMD19-T	E. coli cloning vector	Takara
pET28a	E. coli expression vector	Takara
pET28a-tbrA	TbrA protein expressing plasmid	This work
pEMB0603	E. coli-B. thuringiensis shuttle BAC vector	Ref. 39
pEMB0603-tbrA	pEMB0603 with intact tbrA gene	This work
pEMB0603-tbrB	pEMB0603 with tbrB ORF fused with tbrA gene promoter	This work
pBMB2451	pHT304 carrying tbrA and tbrB genes	This work
pBMB2451-ΔtbrA	tbrA gene deleted pBMB2451 derivative	This work
pBMB2451-ΔtbrB	tbrB gene deleted pBMB2451 derivative	This work
pBMB-tbrB-gfp	pHT304 with tbrB-gfp fusion gene	This work
pBMB-gfp	pHT304 with gfp gene	This work