TABLE 5.
Primers used in this study
Restriction sites and a 10-aa peptide linker-encoding sequence in P15 and P16 primers are underlined.
| Primer and description | Sequence (5′–3′) |
|---|---|
| For amplifying tbr operon | |
| P1 | TATGGATCCGGATACCAAGTTGTAGGAGGTG |
| P2 | GCCGCATGCGCTAATAGGCTTATTGCTTC |
| For deleting tbrA gene | |
| P3 | TATTCTAGATCCTGGTGTAGTGGAAATTCG |
| P4 | GCCTCTAGAGTCATCCCTAAGATTGCGGT |
| For deleting tbrB gene | |
| P5 | TATTCTAGAAGGGACTAAGAATAGAAAAGG |
| P6 | GCCTCTAGAAGAAGGGCAGCAAGTTCA |
| For complementing tbrA gene | |
| P7 | TATGGATCCCCGTGAAGCGTCTCATCCTAG |
| P8 | GCCAAGCTTCTGCTCTTGTCGCACGTCTCG |
| For complementing tbrB gene | |
| P9 | TATGGATCCCCGTGAAGCGTCTCATCCTAG |
| P10 | GTCGCTTTTTTAAGATTATCCATTTATTAGCATCTCCTTTTTATG |
| P11 | CATAAAAAGGAGATGCTAATAAATGGATAATCTTAAAAAAGCGAC |
| P12 | GCCGCATGCGCTAATAGGCTTATTGCTTC |
| For analyzing tbr operon | |
| F1 | GATTAAAGTCGGGTCAGG |
| R1 | CTGCTGATAATGCGAGCG |
| F2 | TCGCTCGCATTATCAGCAG |
| R2 | TGTCGCACGTCTCGTCTTT |
| For amplifying tbrA ORF | |
| P13 | GGAATTCCATATGATGAAAATACCTTGTTTTGTT |
| P14 | GCCCTCGAGAGGTATTATTAATTCGCCTTT |
| For constructing tbrB-gfp fusion gene (also P7, P10, and P11) | |
| P15 | GCCACCTCCGCCTGAACCGCCTCCACCTGATGAACTTGCTGCCCTTCTTTG |
| P16 | TCAGGTGGAGGCGGTTCAGGCGGAGGTGGCATGAGTAAAGGAGAAG |
| P17 | ACATGCATGCTTATTTGTATAGTTCA |
| For identifying pCT6880 of strain CT-43 | |
| P6880-1 | GCTAACAGCATTAGGTGTGC |
| P6880-2 | GCATATGTGGTGTCGCTTC |
| For identifying pCT8252 of strain CT-43 | |
| P8252-1 | CGATTGAAGAGACGTGTAG |
| P8252-2 | CAGAGGATCTCAATCCTAAG |
| For identifying pCT8513 of strain CT-43 | |
| P8513-1 | CGCAACGATGATGGAAGCA |
| P8513-2 | CATTCTACCTTCAGCATCACC |
| For identifying pCT9547 of strain CT-43 | |
| P9547-1 | GGAATGAGATGGTCGAATC |
| P9547-2 | GCATGTAGAGAACGTACAGC |
| For identifying pCT14 of strain CT-43 | |
| P14-1 | TCGTCAGCATTCATTTGAGC |
| P14-2 | GGGTTATCCGTTATATCCTG |
| For identifying pCT51 of strain CT-43 | |
| P51-1 | CACTGGAATGGTGATGTAGA |
| P51-2 | GTATCAGGCATCTTCTGCAC |
| For identifying pCT72 of strain CT-43 | |
| P72-1 | ATCGGTACAACTGGTTCAGG |
| P72-2 | GATGTTGCGGTATGCTAATC |
| For identifying pCT83 of strain CT-43 | |
| P83-1 | GAAGACGGTAATGGATGAAG |
| P83-2 | GCTGCTATACCAATAGACG |
| For identifying pCT127 of strain CT-43 | |
| P127-1 | CAACGAATGTAGTGAACGG |
| P127-2 | CGTCGCTAGGGTAACTATAG |
| For identifying pCT281 of strain CT-43 | |
| P281-1 | GGAGAGTCTGGATTCGT |
| P281-2 | GCGATGTCACCTATCGTTG |