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. 2017 Mar 3;12(3):e0173056. doi: 10.1371/journal.pone.0173056

Fig 6. Reduced H3K9me3 and H4K20me3 formation is seen in cells infected with VACV strain XY-dBID.

Fig 6

The strain designated as XY-dBID-VACV encodes a deletion from N1L to F4L (inclusive). BSC-40 cells were grown on coverslips and infected with VACV or XY-dBID-VACV for 9h or 18h. The cells were fixed and stained with antibodies specific to I3, H3K9me3, or H4K20me3 and for DNA (with DAPI). (A) Representative images showing the staining of nuclear histone markers (scale bar = 25 μm). The amounts of nuclear (B) H3K9me3 and (C) H4K20me3 were measured using FIJI and normalized relative to mock-infected cells. The data represent the SEM of three independent experiments and any statistically significant differences are noted (*P<0.05; **P<0.01; ***P<0.001; **** P<0.0001).