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. 2017 Mar 3;12(3):e0173116. doi: 10.1371/journal.pone.0173116

Fig 5. pON.mCherry functions in several Gram-negative bacterial species.

Fig 5

Plasmids (pMMB207c, black bars; pXDC50, blue bars; pON.mCherry, red bars) were introduced into several Gram-negative bacteria, including (A) E. coli, (B) Acinetobacter baylyi ADP1 and (C) Salmonella typhimurium LT2. The resulting strains were grown for 16 hours in the presence of increasing concentrations of IPTG. mCherry expression is displayed as the mCherry fluorescence divided by optical density to normalize expression values to bacterial growth with error bars representing one standard deviation across three triplicate wells. The experiment was performed in triplicate and data from a representative experiment is shown. (D) Representative photographs showing colonies of the indicated strains harboring pON.mCherry after overnight growth on LB plates at 37°C.