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. 2017 Mar 3;12(3):e0172900. doi: 10.1371/journal.pone.0172900

Fig 4. The effects of leptin on IFN-γ production and T cell-associated alterations in liver samples from patients with NAFLD.

Fig 4

(A) The fold of increase index for IFN-γ production in leptin-stimulated circulating CD4+ cells was higher in patients with NAFLD (n = 10) than in control subjects (n = 10; *p = 0.011). (B) The percentage of CD4+ cells among the total non-parenchymal cell population was higher in patients with NAFLD (n = 10) than in control subjects (n = 10), *p = 0.030. (C) Compared with control subjects (n = 9), patients with NAFLD (n = 9) showed increased hepatic mRNA expression levels of IFN-γ (*p = 0.012), T-bet (*p = 0.020) and CCL20 (*p = 0.007) as measured by quantitative PCR. The 2-ΔΔCt method was used to calculate the mRNA fold change. The box and whiskers indicate the non-parametric statistics: the median, lower and upper quartiles and confidence interval around the median. A two-tailed Mann-Whitney U test was used for the statistical analysis.