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(A) Binding of ATG to CD3Ɛ. BALB/c huCD3Ɛ splenocytes were cultured for 24 h with ATG or control Ig and then stained with anti-huCD3 or anti-mouse CD3 mAbs. Staining was assessed by FACS. (B) Proliferation of CFSE-labeled BALB/c huCD3Ɛ splenocytes after 3 days stimulation with plate-bound ATG, anti-mouse CD3 or anti-huCD3 mAbs, marked by solid line. Representative data of at least 2 independent experiments are shown.
