Table 3. Virulence factor candidates in the N. seriolae UTF1 by comparison with the virulence genes data set of N. farcinica.
| Category | N. seriorae UTF1 ORF ID | N. farcinica gene ID* | Gene* | Description* | %Identity | %Similarity | E value |
|---|---|---|---|---|---|---|---|
| Cell wall proteins | ORF-145 | nfa1810 | fbpA | mycolyltransferase | 70.9 | 79.9 | 5.00E-159 |
| ORF-7590 | nfa1820 | fbpB | mycolyltransferase | 56.4 | 75.9 | 7.00E-67 | |
| ORF-150 | nfa1830 | fbpC | mycolyltransferase | 57.7 | 73.0 | 2.00E-37 | |
| Metal importers | ORF-5098 | nfa37790 | ideR | transcriptional regulator | 66.0 | 79.4 | 4.00E-84 |
| ORF-950 | nfa7630 | nbtA | thioesterase | 85.7 | 90.2 | 0 | |
| ORF-951 | nfa7640 | nbtB | polyketide synthase | 86.4 | 90.0 | 5.00E-60 | |
| ORF-952 | nfa7650 | nbtC | polyketide synthase | 87.3 | 93.0 | 1.00E-143 | |
| ORF-953 | nfa7660 | nbtD | non-ribosomal peptide synthetase | 88.5 | 93.8 | 0 | |
| ORF-954 | nfa7670 | nbtE | non-ribosomal peptide synthetase | 91.6 | 96.2 | 4.00E-161 | |
| ORF-750 | nfa7680 | nbtF | non-ribosomal peptide synthetase | 93.0 | 97.1 | 2.00E-108 | |
| ORF-948 | nfa7610 | nbtG | lysine-N-oxygenase | 84.7 | 93.1 | 0 | |
| ORF-747 | nfa6190 | nbtS | salicylate synthase | 81.4 | 88.5 | 4.00E-126 | |
| ORF-749 | nfa6200 | nbtT | salicylate-AMP ligase | 82.5 | 91.3 | 4.00E-126 | |
| Oxidative and nitrosative stresses | ORF-5118 | nfa37890 | ahpC | alkylhydroperoxide reductase | 68.0 | 79.0 | 5.00E-149 |
| ORF-5119 | nfa37900 | ahpD | alkylhydroperoxidase | 68.8 | 79.3 | 0 | |
| ORF-325 | nfa55390 | katC | catalase | 79.0 | 87.8 | 0 | |
| ORF-4212 | nfa29500 | katG | catalase-peroxidase | 63.8 | 77.8 | 1.00E-169 | |
| ORF-6237 | nfa45490 | narG | nitrate reductase alpha subunit | 71.3 | 84.8 | 0 | |
| ORF-6238 | nfa45500 | narH | nitrate reductase beta subunit | 72.4 | 83.3 | 1.00E-78 | |
| ORF-6240 | nfa45520 | narI | nitrate reductase gamma subunit | 75.3 | 84.6 | 0 | |
| ORF-6239 | nfa45510 | narJ | nitrate reductase delta subunit | 74.1 | 83.8 | 1.00E-157 | |
| ORF-6265 | nfa45610 | nirB | nitrite reductase (NAD(P)H) subunit | 76.9 | 86.9 | 0 | |
| ORF-6264 | nfa45600 | nirD | nitrite reductase (NAD(P)H) subunit | 75.6 | 84.0 | 1.00E-173 | |
| ORF-5117 | nfa37880 | oxyR | hydrogen peroxide sensing transcriptional regulator | 66.9 | 78.6 | 2.00E-165 | |
| ORF-418 | nfa52980 | sodC | superoxide dismutase | 78.2 | 86.4 | 1.00E-140 | |
| ORF-68 | nfa1210 | sodF | superoxide dismutase | 40.5 | 57.5 | 3.00E-112 | |
| Penetration into mammalian cells | ORF-4357 | nfa34810 | inv | invasin | 65.6 | 80.4 | 2.00E-119 |
| Phagosome arresting | ORF-5937 | nfa13510 | ndk | nucleoside diphosphate kinase | 44.8 | 57.6 | 2.00E-45 |
| ORF-5498 | nfa16310 | ptpA | protein-tyrosine phosphatase | 48.7 | 61.4 | 2.00E-88 | |
| ORF-2644 | nfa18680 | ptpB | protein-tyrosine phosphatase | 60.2 | 70.7 | 0 | |
| other | ORF-2873 | nfa19960 | tlyA | putative cytotoxin/hemolysin | 77.4 | 83.2 | 6.00E-135 |
* according to the Nocardia farcinica Genome Project Page (http://nocardia.nih.go.jp/).