Fig 5. Non-constant and constant-ratio data for the atovaquone-proguanil combination.

Ring stage parasites were treated for 72 hours with constant and non-constant ratio combinations of atovaquone and proguanil. Parasitaemia was determined using the SG-FCM method (a and c). After 72 hours of treatment, drug pressure was removed; parasites were subjected to 8 10-fold limiting serial dilutions and incubated for a further 17 days. Spent media was replaced with fresh media and erythrocytes on day 7. On day 20, the presence or absence of growth in each well was determined using SC-FCM and used to back calculate parasite viability after the 17 day recovery period (b and d). Triplicate data was used to construct error bars that represented the standard error of the mean (SEM).