Fig 2. ITC analysis of Pr55^Gag-RNA binding show more energetically favourable binding of Pr55^Gag to A-containing over GU-containing RNA.

(A) Schematic of Pr55^Gag-RNA binding specificity during viral assembly. Representative ITC binding curves indicating heat released per mole of oligonucleotide titrated when 40 μM of 20mers; (B) 4x 5’-GAUGG-3’ (GU-containing) and 4x 5’-GAGAA-3’ (A-containing) RNA (n = 3);.and (C) 4x 5’-UGUGG-3’ (GU-containing) and 4x 5’-AAGGA-3’ (A-containing) RNA were injected in 1.5 ul aliquots into 8 μM of Pr55^Gag, Pr50^GagΔp6, Pr55^{Gag WM}, p15^NC-SP2-p6 and p7^NC, respectively (n = 2).