Table 1. CLAMS metabolic cage results.
Time (light and dark cycle) had a significant effect on all parameters. No significant effect of strain or interaction was found for other parameters measured in metabolic profiling cages.
| Control | Cau-3 OE | Statistics | F (DFn, DFd) | P value | |||
|---|---|---|---|---|---|---|---|
| Weight (grams) | Before | 25.94 ± 1.77 | 25.82 ± 2.49 | 2-Way RM ANOVA | Time | F (1, 10) = 12.65 | P = 0.005 |
| After | 24.52 ± 1.53 | 25.12 ± 2.63 | Strain | F (1, 10) = 0.04 | P = 0.845 | ||
| Interaction | F (1, 10) = 1.482 | P = 0.252 | |||||
| t (df) |
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| Change | −1.42 ± 1.12 | −0.70 ± 0.93 | t test | unpaired | t (10) = 1.217 | P = 0.252 | |
| VO2 (ml/kg/hr) | Dark 1 | 4488.15 ± 219.92 | 4402.29 ± 255.72 | 2-Way RM ANOVA | Time | F (4, 40) = 63.89 | P < 0.001 |
| Light 1 | 3965.26 ± 270.28 | 3894.60 ± 267.46 | Strain | F (1, 10) = 0.086 | P = 0.776 | ||
| Dark 2 | 4691.70 ± 229.34 | 4643.84 ± 361.87 | Interaction | F (4, 40) = 0.499 | P = 0.737 | ||
| Light 2 | 3979.32 ± 179.73 | 3905.65 ± 249.86 | |||||
| Dark 3 | 4495.64 ± 365.01 | 4558.41 ± 393.24 | |||||
| VCO2 (ml/kg/hr) | Dark 1 | 4057.02 ± 196.67 | 3978.93 ± 345.03 | 2-Way RM ANOVA | Time | F (4, 40) = 47.79 | P < 0.001 |
| Light 1 | 3440.59 ± 263.67 | 3476.22 ± 253.98 | Strain | F (1, 10) < 0.001 | P = 0.998 | ||
| Dark 2 | 4289.97 ± 185.37 | 4278.28 ± 345.75 | Interaction | F (4, 40) = 0.166 | P = 0.954 | ||
| Light 2 | 3322.22 ± 142.58 | 3365.32 ± 205.61 | |||||
| Dark 3 | 4076.90 ± 468.88 | 4085.79 ± 442.01 | |||||
| RER | Dark 1 | 0.90 ± 0.02 | 0.90 ± 0.04 | 2-Way RM ANOVA | Time | F (4, 40) = 9.095 | P < 0.001 |
| Light 1 | 0.87 ± 0.02 | 0.89 ± 0.02 | Strain | F (1, 10) = 1.517 | P = 0.246 | ||
| Dark 2 | 0.91 ± 0.02 | 0.92 ± 0.02 | Interaction | F (4, 40) = 0.732 | P = 0.576 | ||
| Light 2 | 0.83 ± 0.04 | 0.86 ± 0.01 | |||||
| Dark 3 | 0.90 ± 0.05 | 0.89 ± 0.05 | |||||
| Heat (kcal/hr) | Dark 1 | 0.57 ± 0.05 | 0.56 ± 0.04 | 2-Way RM ANOVA | Time | F (4, 40) = 70.52 | P < 0.001 |
| Light 1 | 0.50 ± 0.06 | 0.49 ± 0.04 | Strain | F (1, 10) = 0.075 | P = 0.789 | ||
| Dark 2 | 0.60 ± 0.05 | 0.59 ± 0.04 | Interaction | F (4, 40) = 0.198 | P = 0.938 | ||
| Light 2 | 0.50 ± 0.04 | 0.49 ± 0.03 | |||||
| Dark 3 | 0.58 ± 0.06 | 0.58 ± 0.04 | |||||
| Feeding, accumulated (grams) | Dark 1 | 2.36 ± 0.82 | 1.97 ± 0.61 | 2-Way RM ANOVA | Time | F (4, 40) = 1471 | P < 0.001 |
| Light 1 | 5.31 ± 1.66 | 4.58 ± 0.65 | Strain | F (1, 10) = 0.991 | P = 0.343 | ||
| Dark 2 | 8.05 ± 1.86 | 7.21 ± 0.77 | Interaction | F (4, 40) = 0.681 | P = 0.609 | ||
| Light 2 | 10.28 ± 1.70 | 9.54 ± 0.86 | |||||
| Dark 3 | 12.76 ± 1.56 | 12.08 ± 0.75 | |||||
| Drinking, accumulated (ml) | Dark 1 | 1.48 ± 0.33 | 1.66 ± 0.43 | 2-Way RM ANOVA | Time | F (4, 40) = 1037 | P < 0.001 |
| Light 1 | 3.97 ± 0.54 | 3.98 ± 0.59 | Strain | F (1, 10) = 0.025 | P = 0.878 | ||
| Dark 2 | 6.22 ± 0.68 | 6.18 ± 0.52 | Interaction | F (4, 40) = 0.135 | P = 0.969 | ||
| Light 2 | 8.39 ± 0.87 | 8.43 ± 0.63 | |||||
| Dark 3 | 10.57 ± 1.17 | 10.65 ± 0.76 | |||||
Data are Mean ± SD. Significance was assumed when p < 0.05. VO2: oxygen consumption; VCO2: carbon dioxide elimination; RER: respiratory exchange ratio