Figure 1.

Pre-storage experimental filtration removes 5-lipoxygenase activating protein (FLAP) and 5-lipoxygenase (5-LO) from red blood cell (RBC) units.

(A) There is significant FLAP immunoreactivity prior to filtration in day 1 (Pre D1) supernatants that is removed by filtration (Post D1) which re-accumulates during routine storage by day 42, the end of storage (Post D42). The supernatants from two different RBC units were used. (B) There is significant reactivity for 5-lipoxygenase (5-LO) both before (Pre) and after (Post) leucoreduction and at the end of storage (D42) with the Haemonetics BPF4 filter. The 5-LO immunoreactivity is present prior to experimental filtration (Pre) that is removed from the supernatant of 2 separate RBC units that does not re-accumulate during routine storage D42. Figure representative of 3 separate experiments which demonstrated similar results.