Table 1.

Requirement of data for Act on the Registration and Evaluation of Chemicals registration

Field	Tonnage [no. of test]a
	≥1-10< [15]	≥10-100< [26]	≥100-1000 < [37]	≥1000 [47]
Physicochemical property	State of substance	Flammability	Viscosity
	Water sdubility	Explosiveness	Dissociation constant
	Melting/freezing point	Oxidation
	Boiling point
	Vapor pressure
	Octanol/water partitioning coefficient
	Density
	Particle size analysis
Hazard on human health	Acute toxicity-oral (acute toxicity-inhalation)	Acute toxicity-dermal route or inhalation	Additional genotoxicity test (reproductive cell and genotoxicity)	Repeated dose toxicity study (90 d)
				Teratogenicity
	Ames test (bacterial reverse mutation assay)	Eye irritation/corrosion		Two-generation reproductive toxicity
		Chromosomal aberration test		Carcinogenicity
	Skin irritation/corrosion	Genotoxicity test
	Skin sensitization	Repeated dose toxicity study (28 d)
		Screening for reproductive/ developmental toxicity
Hazard on the environment	Acute toxicity on fish	Fresh water algae growth inhibition test	Inherent biodegradability	Further information on the environmental fate and behavior
	Ready biodegradability		Identification of degradation products
	Acute daphnia immobilization test	Biodegradation as a function of pH	Chronic toxicity on fish	Chronic toxicity on terrestrial plants
			Chronic toxicity on preferred species daphnia	Chronic toxicity testing on terrestrial invertebrates
			Acute toxicity on terrestrial plants	Further information on adsorption/ desorption depending on the results of the study
			Activated sludge respiration inhibition Adsorption/desorption
				Long-term toxicity to sediment organisms
				Bio-concentration

^aTest items for lower tonnage are also required.