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. Author manuscript; available in PMC: 2017 Sep 2.
Published in final edited form as: Oncogene. 2016 Aug 29;36(9):1309–1314. doi: 10.1038/onc.2016.298

Figure 2.

Figure 2

Conditional deletion of IR-induced p16INK4a expression in mice. 8–10 weeks old mice were irradiated at the dose of 2.5 Gy (total body irradiation using a Faxitron CP-160 at a rate of 1 Gy/min) and 8 weeks later they were treated (+) or not (−) with Tamoxifen (Tam) at a dose of 200 mg/kg (diluted in a mixture 1:50 of ethanol and corn oil respectively) by gavage for 5 consecutive days to inactivate p16INK4a. Expression of p16INK4a relative to 18S was determined by qPCR on liver and spleen tissues collected from Cre p16L/L (a) or p16L/L (b) mice. n=5 mice per group. Data are expressed as mean ± SD. Student t-test * p < 0.05 and ** p < 0.01.