Table 2.
Solvent | Fraction | TPC (mg GAE/g) | TFC (mg CE/g) | TAA (μmol AAE/g) | FRP (μmol AAE/g) | DPPH (%) | |||||
---|---|---|---|---|---|---|---|---|---|---|---|
Conventional | Ultrasound | Conventional | Ultrasound | Conventional | Ultrasound | Conventional | Ultrasound | Conventional | Ultrasound | ||
Acetone | Hull | 63.29 ± 0.62n | 79.65 ± 0.83o | 0.86 ± 0.01i | 1.40 ± 0.05l | 66.70 ± 0.90n | 98.85 ± 0.73o | 48.92 ± 0.88n | 57.11 ± 0.74o | 76.75 ± 0.69m | 86.31 ± 0.57p |
Cotyledon | 9.04 ± 0.10ab | 15.33 ± 0.71fg | 0.14 ± 0.01a | 0.18 ± 0.01abc | 25.19 ± 0.66b | 43.01 ± 0.79hi | 6.09 ± 0.27ab | 6.58 ± 0.13abc | 20.12 ± 0.66b | 32.61 ± 0.14e | |
Whole | 19.68 ± 0.22h | 31.31 ± 0.68k | 0.26 ± 0.01c | 0.55 ± 0.01ef | 35.70 ± 0.67d | 51.93 ± 0.60l | 7.87 ± 0.36cde | 11.91 ± 0.67gh | 28.13 ± 0.40d | 65.27 ± 0.35l | |
Ethanol | Hull | 37.74 ± 0.27l | 42.22 ± 0.70m | 0.85 ± 0.01i | 1.13 ± 0.08k | 52.46 ± 0.55l | 55.46 ± 0.55m | 28.22 ± 0.56l | 33.06 ± 0.44m | 81.30 ± 0.43n | 83.59 ± 0.32o |
Cotyledon | 8.65 ± 0.48ab | 13.31 ± 0.68de | 0.13 ± 0.01a | 0.16 ± 0.01ab | 30.84 ± 0.72c | 41.12 ± 0.81gh | 7.42 ± 0.22bcd | 11.30 ± 0.42gh | 12.82 ± 0.43a | 32.15 ± 0.47e | |
Whole | 20.23 ± 0.62h | 25.87 ± 0.85j | 0.48 ± 0.01e | 0.71 ± 0.04h | 42.96 ± 0.94hi | 47.45 ± 0.85k | 8.20 ± 0.48de | 15.07 ± 0.49i | 44.29 ± 0.56h | 50.23 ± 0.39j | |
Methanol | Hull | 31.48 ± 0.50k | 36.81 ± 0.88l | 0.67 ± 0.01gh | 0.97 ± 0.06j | 50.81 ± 0.65l | 55.52 ± 1.17m | 21.54 ± 0.55j | 24.41 ± 0.61k | 80.87 ± 0.24n | 83.21 ± 0.40o |
Cotyledon | 8.64 ± 0.47ab | 11.60 ± 0.59cd | 0.13 ± 0.00a | 0.16 ± 0.01ab | 36.13 ± 0.92d | 38.75 ± 0.90ef | 6.16 ± 0.21ab | 9.20 ± 0.10ef | 29.25 ± 0.41d | 36.49 ± 0.48f | |
Whole | 19.89 ± 0.66h | 22.92 ± 0.76i | 0.35 ± 0.01d | 0.61 ± 0.03fg | 40.38 ± 0.50fg | 44.67 ± 0.69ij | 8.90 ± 0.44de | 12.10 ± 0.42h | 39.88 ± 0.44g | 48.72 ± 0.34i | |
Water | Hull | 16.05 ± 0.47g | 31.85 ± 0.79k | 0.24 ± 0.00bc | 0.92 ± 0.04ij | 29.60 ± 0.61c | 46.30 ± 0.68jk | 11.67 ± 0.33gh | 20.90 ± 0.68j | 61.63 ± 0.38k | 75.43 ± 0.58m |
Cotyledon | 7.62 ± 0.30a | 10.20 ± 0.81bc | 0.11 ± 0.01a | 0.17 ± 0.01ab | 18.56 ± 0.62a | 30.53 ± 0.64c | 5.77 ± 0.23a | 7.91 ± 0.46cde | 12.77 ± 0.24a | 25.23 ± 0.44c | |
Whole | 14.06 ± 0.55ef | 20.49 ± 0.86h | 0.17 ± 0.01ab | 0.39 ± 0.01d | 25.24 ± 0.70b | 37.55 ± 0.74de | 7.54 ± 0.32bcd | 10.56 ± 0.55fg | 36.42 ± 0.39f | 49.54 ± 0.42ij |
Values expressed as mean (n = 3) ± standard deviation; Different letters in the same variable sub-columns represent statistically different results according to the Fisher HSD test (p < 0.05)
TPC total phenolic content, TFC total flavonoids content, TAA total antioxidant activity, FRP ferric reducing power, DPPH 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity, GAE gallic acid equivalent, CE catechin equivalent, AAE ascorbic acid equivalent