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. Author manuscript; available in PMC: 2018 Mar 3.
Published in final edited form as: J Proteome Res. 2017 Feb 3;16(3):1121–1132. doi: 10.1021/acs.jproteome.6b00374

Table 4.

Coverage compared with published proteome and phosphoproteome datasets

Source Organism Starting
material
per
channel
Fractions LC-
MS/MS
per
replicate
Protein
coverage
Phosphorylation
sites coverage
Quantitation
method
Instrument
This study Human
breast
cancer cell
lines
0.67
mg
12 TiO2 2h ID:
8706

Q: 7394
ID: 28186

Q: 23739
TMT10plex-
MS2
QE-HF
Paulo et al. Human
pancreatic
stellate
cells
5 mg 12 TiO2 3h ID: NA

Q: 8120
ID: 21556

Q: 16622
TMT9plex-
MS3
Orbitrap
Fusion
Radhakrishnan
et al.
Human
head and
neck

squamous
cell
carcinoma
NA 12 TiO2 NA ID:
3255

Q: NA
ID: 4920

Q: NA
TMT7plex LTQ-
Orbitrap
Velos
Paulo et al. Mouse
Kidney,
Liver, and
Pancreases
tissues
10 mg 12 TiO2 3h ID:
7900

Q: NA
ID: NA

Q: 10562
TMT10plex-
MS3
Orbitrap
Fusion
Hoffman et al. Human
muscle
peptides
NA NA 100
min
Q: 4317 Q: 11903 TMT4plex QE
Erickson et al. Murine
brain and
liver
tissues
10 mg 12 TiO2 3h NA Q: 11015 TMT10plex Orbitrap
Fusion