Figure 4.

WZ35 inhibits colon cancer tumor xenograft growth in vivo. A. Tumor volumes in WZ35 and curcumin treated mice were smaller than those of vehicle treated mice. CT26 cells were injected to the flaks of Balb/C mice and the tumors were allowed to develop for 14 d. Subsequently, Balb/C mice bearing CT26 xenografts received WZ35 orally at the dose of 25 or 50 mg/kg or 50 mg/kg curcumin for a total of 14 days. Tumor volume was monitored. B, C. On day 14, tumors were excised and subjected to weight analysis. D. Body weight of the Balb/C mice in vehicle treated, WZ35 treated and curcumin treated mice. E. Tumor sections were performed for TUNEL staining analysis to detect apoptotic cells. F. Western blot analysis on the expressions of caspase 3, GAPDH was used as protein loading control, and tumor sections were stained with an anti-Cle-PARP Ab to detect PARP cleavage from respective tumor tissue. *p<0.05; **p<0.01, vs control group.