Skip to main content
. 2017 Feb 1;7(2):275–288.

Figure 5.

Figure 5

WZ35 induces cytotoxicity in colon cancer cells is dependent on intracellular ROS generation and subsequent ER stress activation. A. Intracellular ROS generation induced by increasing doses of WZ35 was measured in CT26 cells by staining with DCFH-DA (10 μM) and flow cytometry analysis. B. CT26 cells were pre-incubated with 5 mM NAC for 1 h before exposure to WZ35 (10 M) for 8 h. Intracellular ROS generation was measured by flow cytometry. C. Blocking of ROS generation CT26 Cells were pre-incubated with or without 5 mM NAC for 1 h before exposure to WZ35 (10 μM) for 24 h. Cell shape was observed. D. CT26 cells were pretreated with or without 5 mM NAC for 1 h before exposure to WZ35. Percentage of cell apoptosis was determined by Annexin-V/PI staining and flow cytometry. E. Effect of WZ35 on the morphology of endoplasmic reticulum in CT26 cells. Cells were pre-incubated with or without 5 mM NAC for 1 h before exposure to WZ35 (10 μM) for 4 h. The morphology of endoplasmic reticulum in CT26 cells was examined with an electron microscope (× 10000 or × 30000). F. Effect of WZ35 on CHOP expression in CT26 cells. Cells were pre-incubated with or without 5 mM NAC for 1 h before exposure to WZ35 (10 μM) for 12 h. The fluorescence of CHOP was observed through immunostaining and fluorescence microscope.