Figure 6.

ER stress is involved in WZ35-induced colon cancer cells apoptosis. A. CT26 cells were treated with WZ35 (10 μM) for different time, the protein levels of P-eIF2α, P-PERK, ATF4 and CHOP were determined by western blot. B. CT26 cells were treated with WZ35 (2.5, 5 or 10 μM) or curcumin (20 μM) for indicated time, the expression of p-eIF2α, p-PERK, ATF4 and CHOP were detected by western blot. GAPDH was used as internal control. C. CT26 cells were pre-incubated with 5 mM NAC for 1 h before treated with WZ35 (10 μM) for 4 or 12 hours. The expression of ATF-4 or CHOP was detected by western blot assay. D. CT26 cells were incubated with p-PERK inhibitor (GSK2606414), the expression of p-PERK or CHOP in CT26 cells was determined by western blot after stimulation with WZ35 (10 μM) for 2 h or 12 h. E. CT26 cells were pre-incubated with 2 μM GSK2606414 for 2 h before treated with WZ35 or were only treated with WZ35 for 24 h. Cell viability was determined by MTT assay.