Figure 5.

PI3K/AKT pathway activity is associated with CHIP/miR-92b/PTEN axis. A. Western blot analysis is performed to detect activity of several proteins involved in PI3K/AKT pathway when CHIP or PTEN inhibition or miR-92b overexpression; GAPDH is used as an internal control. B. AKT expression and its activity in LN229 cells are analyzed by Western blot when AKT and miR-92b expression are changed together or separately. C, D. In different conditions of AKT and miR-92b expression pattern, proliferation ability and apoptosis of LN229 cells are measured by MTT assay and flow cytometry. AKT inhibition offsets the effect on glioma cell growth induced by miR-92b overexpression, and re-introduction of miR-92b can abrogate the AKT inhibition induced effect on glioma cell apoptosis (*P<0.05, miR-92b+Akt siRNA vs. miR-92b+siR-con; miR-92b+Akt siRNA vs. miR-con+Akt siRNA).