Table 2.
Cross-reactive Serum Microneutralization Titers Against Heterologous Influenza A Viruses
| Virus | Subtype/Lineage | Host Species | MN Titer and Vaccine Formulationa |
||||
|---|---|---|---|---|---|---|---|
| Naïve | Monovalent Prime Only | Monovalent Prime & Boost | Trivalent Prime Only | Trivalent Prime and Boost | |||
| A/Japan/305/1957 | H2N2 (pandemic) | Human | < | < | 10 | 20 | 20 |
| A/AnnArbor/23/1957 | H2N2 (pandemic) | Human | ND | ND | ND | ND | ND |
| A/Netherlands/84/1968 | H2N2 (seasonal) | Human | < | < | 20 | 40 | 40 |
| A/NorthCarolina/1/68 | H2N2 (seasonal) | Human | < | < | 40 | 40 | 40 |
| Human H2 GMT (Log2)b | 0.0 | 0.0 | 3.2 | 4.0 | 4.0 | ||
| A/Mallard/MT/Y61 | H2N2 | Avian | < | < | 10 | 20 | 40 |
| A/Mallard/Potsdam/179/1983 | H2N2 | Avian | < | 20 | 40 | 320 | 320 |
| A/Chicken/Jena/4836/1983 | H2N2 | Avian | < | < | 10 | 10 | 40 |
| A/Mallard/NewYork/6750/1978 | H2N2 | Avian | < | < | 20 | 40 | 40 |
| A/Chicken/Connecticut/13657/1990 | H2N2 | Avian | < | < | 40 | 40 | 80 |
| A/GuineaFowl/NewYork/13824/1995 | H2N2 | Avian | < | < | 10 | 10 | 160 |
| A/Chicken/Ohio/494832/2007 | H2N3 | Avian | < | < | 10 | 20 | 40 |
| A/Widgeon/Denmark/66174/G18/2004 | H2N3 | Avian | < | < | 10 | 20 | 40 |
| A/Mallard/Alberta/35/2009 | H2N3 | Avian | < | < | 10 | 20 | 20 |
| Avian H2 GMT (Log2)b | 0.0 | 0.2 | 2.8 | 3.6 | 4.8 | ||
| A/Brisbane/10/2007c | H1N1 (pre-pandemic) | Human | < | < | < | < | < |
| A/California/04/2009c | H1N1 (pandemic) | Human | < | < | < | < | < |
| A/Perth/16/2009c | H3N2 (seasonal) | Human | < | < | < | < | < |
| rgA/Egypt/N03072/2010c, d | H5N1 (clade 2.2.1) | Human | < | < | < | < | < |
| rgA/Guizhou/1/2013(H5N1)c, d | H5N1 (clade 2.3.4.2) | Human | < | < | < | < | < |
< Below the assay limit of detection (1:10 serum dilution), GMT - Geometric Mean Titer, ND - not determined
a
Individual MN values are expressed as the reciprocal of the highest dilution that inhibited 100 TCID50 units of the indicated test virus with horse erythrocytes unless otherwise specified.
b
GMT was determined by assigning 1 (Log2) values for samples below the assay limit of detection and subtracting 1 from the final GMT
c
MN assays were performed with chicken erythrocytes
d
Represents reverse genetics (rg) vaccine viruses containing HA and NA surface proteins on A/Puerto/Rico/8/1934 virus backbone