Figure 6.

Effects of mexedrone, N-methoxymephedrone, α-chloromethylmephedrone and comparison test drug mephedrone on inhibition of uptake and stimulation of release at DAT, NET and SERT in rat brain synaptosomes. For uptake assays (A, B and C), synaptosomes were incubated with different concentrations of the test drugs in the presence of 5 nM [³H]dopamine (A, for DAT), 10 nM [³H]norepinephrine (B, for NET) or 5 nM [³H]serotonin (C, for SERT). Data are expressed as percentage of [³H]transmitter uptake (mean± SD) for n =3 experiments. For release assays (D, E and F), synaptosomes were preloaded with 9 nM [³H]MPP⁺ for DAT (D) and NET (E) or 5 nM [³H]serotonin for SERT (F), then incubated with different concentrations of test drugs to evoke release via reverse transport. Data are expressed as percentage of [³H]substrate release (mean ± SD) for n = 3 experiments.