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. 2017 Feb 17;49(2):e294. doi: 10.1038/emm.2016.147

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Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Effect of VCAM-1-D6 protein on HUVEC tube formation. (a) Light microscopic images depicting tube formation by hTNFα-treated HUVECs in the absence (MOCK) or presence of the indicated concentrations of VCAM-1-D6-Fc fusion protein or Fc domain alone. Fc served as a negative control. (b) Quantitation of total tube branches in hTNFα-treated HUVECs in presence of VCAM-1-D6-Fc or Fc, expressed as a percent of control (MOCK) tube formation. All data are presented as the mean±s.e.m. of triplicate measurements from one of the three independent experiments; ***P<0.001.