Figure 2.

Antagonism of CXCR3 inhibits CXCL10 expression in 4T1 cells. (a) 4T1 cells were cultured for the indicated times, and secreted CXCL10 levels were measured by ELISA (*P<0.05, **P<0.01). (b, c) 4T1 cells were cultured for 24 h with DMSO or various doses of JN-2. After culturing, cell viability (b) and CXCL10 mRNA levels (c) were analyzed by the EZ-Cytox assay kit and real-time PCR, respectively (*P<0.05). (d) 4T1 cells were cultured with DMSO or JN-2 (10 μM) for 12 or 24 h, and CXCL10 production was measured (*P<0.05, **P<0.01). (e) 4T1 cells were cultured with various doses of mCXCL10 for 24 h, CXCL10 mRNA levels were analyzed (*P<0.05). (f) 4T1 cells were cultured with mCXCL10 (300 ng ml⁻¹) alone or together with JN-2 (10 μM) for 24 h, and CXCL10 mRNA levels were analyzed (*P<0.05).