Figure 3.

Resveratrol (REV) suppresses lysophosphatidic acid (LPA)- and epidermal growth factor (EGF)-induced breast cancer cell invasion. (a, b) The serum-starved MDA-MB-231 and MDA-MB-468 cells were pretreated with REV (25 μM) for 1 h and in vitro invasion and migration were analyzed against LPA (5 μM) (a) and EGF (100 ng ml⁻¹) (b) (***P<0.001 versus control, ^###P<0.001 versus LPA and EGF treatment only). (c) The MDA-MB-231 cells were transfected with the indicated siRNA. Then, the cells were pretreated with REV (25 μM) for 1 h, followed by stimulation with or without LPA (5 μM; ***P<0.001 versus control, ^##P<0.01 versus LPA treatment only, ^###P<0.001 versus LPA treatment only, ^$$P<0.01 versus LPA treatment and YAP siRNA). Original magnification × 200. (d) The MDA-MB-231 cells were transfected with the indicated siRNA and then stimulated with or without LPA (5 μM; ***P<0.001 versus control, ^#P<0.05 versus LPA treatment only, ^##P<0.01 versus LPA treatment only, ^$$P<0.01 versus TAZ siRNA and LPA treatment). Original magnification of all images, × 200. All experiments were repeated three times.