Table 4.
Primers designed for qRT-PCR
| 5′–3′ (Forward) | 5′–3′ (Reverse) | |
|---|---|---|
| Liver | ||
| 11β-HSD1 | GAAGAAGCATGGAGGTCAAC | GCAATCAGAGGTTGGGTCAT |
| GR | AGGGGAGGGGGAGCGTAATGG | CCTCTGCTGCTTGGAATCTGC |
| MR | ACCACATACATCGCTCCGAC | ATGGTGTGAGTGCATGCGTA |
| PEPCK | CTTCCCTCTGGGAACACACC | GTGCCACCTTTCTTCCTCCT |
| PC | CCAACATCCCATTCCAGATGC | CCACCTCACAGAACTTGAAGAC |
| G6Pase | AAGTCGCTCCCATTCCGTTT | GGGCTTCAGCGAGTCAAAGA |
| gWAT | ||
| GLUT4 | TGCAGTGCCTGAGTCTTCTT | GGTTCCCCATCTTCAGAGCC |
| PCG-1α | CATTCAGGAGCTGGATGGCT | CAAAGAGGCTGGTCCTCACC |
| IR | TGCTGAGGACACTAGGCCAT | GGGTGTAGTGGCTGTCACAT |
| Housekeeper | ||
| GAPDH | TCTCTGCTCCTCCCTGTTCT | TACGGCCAAATCCGTTCACA |
11β-HSD1, 11β-hydroxysteroid dehydrogenase type 1; GR, glucocorticoid receptor; MR, mineralocorticoid receptor; PEPCK, phosphoenolpyruvate carboxykinase; PC, pyruvate carboxylase; G6Pase, glucose-6-phosphatase; gWAT, gonadal white adipose tissue; GLUT4, glucose transporter 4; PGC-1α, peroxisome proliferator-activated receptor-γ coactivator-1α; IR, insulin receptor.