Fig. 6.

DNAH5 knockdown recapitulates PCD phenotype. A: qRT-PCR was used to assess DNAH5 mRNA expression in NHBE-BMI-1 cells and NHBE BMI-1-transduced with lentivirus expressing either a scrambled or DNAH5-targeting shRNA and grown in submerged 2D culture. **P < 0.01; 1-way ANOVA with Bonferroni’s posttest used to assess significance. Data are means ± SE. B: immunostaining for DNAH5 and acetylated α-tubulin was used to assess the presence or absence of DNAH5 in the ciliary axoneme of shRNA-transduced and untransduced NHBE BMI-1 cells differentiated at ALI. Presence of GFP fluorescence denotes cells transduced with the GFP-shRNA construct and so expressing the shRNA. Scale bars are 20 µm. Images are representative of 4 independent ALI cultures per condition.