Fig. 1.

Percent recovery of recombinant IL-8 from Leukosorb strips using various storage conditions. A: flowchart describing methods for detecting the percent recovery of IL-8 recombinant protein from Leukosorb strips. B: image of a Leukosorb strip and its relative size. C: diagram and measurements of cut Leukosorb strips. D: percent recovery of recombinant IL-8 from Leukosorb strips at varying storage conditions (n = 5). A known amount of recombinant IL-8 with no carrier was spiked onto Leukosorb strips. The strips were then stored in triplicate in four storage conditions (immediate elution, −20°C, room temperature, or 37°C). Protein was then eluted from strips and the eluant was stored at −20°C until analysis (at least 24 h). The strips were then batch-analyzed by ELISA and compared with protein of a known concentration. Compared with the strips that were immediately eluted, there was a significant difference in percent recovery only in the 37°C group. Values are presented as means ± SE. *P ≤ 0.05.