Fig. 5.

Expression of sirtuin 1 (SIRT1) in diabetes. A: SIRT1 expression in kidney lysates obtained from db/db and dbm mice at ages 12 (time of a shift from normoglycemia to hyperglycemia) and 30 (time of persistent hyperglycemia) wk. Note that at 30 wk the expression of Sirt1 is reduced. The panel on the bottom summarizes these Western blotting studies. *P < 0.05 (unpublished observations). B: left, results of Western blot analysis of Sirt1 expression after its overexpression (SIRT1), inhibition (shRNA), and control transfection (Mock). Right, results of Sirt1 overexpression or suppression on apoptosis and senescence in cells subjected to GC or NC. *P < 0.05. Reprinted with permission from Ref. 9.