Fig. 7.

Analysis of bone marrow-derived endothelial progenitors. A-C: fluorescence-activated cell sorting (FACS) analysis (A and C) and direct cell culture of bone marrow-derived cells (B) indicate an increase in the proportion of apoptotic progenitors and a decrease in the number of progenitors detected through lectin⁺/LDL⁺ cells or Sca1⁺/Flk1⁺ cells in db/db mice. Pretreatment of db/db mice in vivo or db/db mice-derived bone marrow cells ex vivo with the organoselenic antioxidant and peroxinitrite scavenger ebselen results in improved viability and restored numbers of bone marrow-derived progenitors. P < 0.05 compared with db/m (*) and with TxCM (#). Reprinted with permission from Ref. 12.